This is a spread from AMAZING SPIDER-MAN #655 by writer Dan Slott & artist Marcos Martin. The issue is easily one of my favorite Spidey comics ever. Really emotional and just insanely gorgeous.
Anyway, these two pages feature Spidey and a ton of deceased Spider-Man allies. On Marvel.com, Associate Editor Ben Morse posted a comprehensive guide to who everyone in the spread is, along with some info on each. Really cool stuff, check it out.